Master Student Analytical Characterization (d/f/m) temporary for 6 months

Job Description Summary

Sandoz is going through an exciting and transformative period as a global leader and pioneering provider of sustainable Generic and Biosimilar medicines.
Now as an independently listed company, Sandoz aims to increase its strategic focus, operate with greater agility, set clearer business objectives, enhance shareholder returns, and strengthen its culture for us, the Sandoz associates. This is an exciting time in our history, and by creating a new and ambitious path, it will provide a unique opportunity for us all, both professionally and personally.
Join us as a Founder of our ‘new’ Sandoz!
Master thesis with the topic:
Development of a robust reducing peptide mapping workflow to characterize mAb via multi-attribute method (MAM)
The multi-attribute-method (MAM) is a liquid chromatography-mass spectrometry (LC-MS) based method that is used to comprehensively characterize and monitor numerous critical quality attributes (CQAs) at the amino acid level of a biopharmaceuticals. The MAM is based on the reduced peptide mapping (rPepmap) workflow, encompassing the in-solution disulfide linkage reduction and alkylation, proteolytic digestion of proteins into peptides followed by reversed-phase LC separation and MS detection and identification. To develop a reliable MAM, an optimized rPepmap workflow needs to be implemented to increase the robustness and the reproducibility of LC-MS data for identification and relative quantification of CQAs.

Job Description

Your Key Responsibilities:

• Optimization of sample preparation for protein digestion to reduce methionine related method artefacts, and to reduce the efforts for the regeneration of column, as well as to reduce the misscleaved or nonspecific cleaved peptide abundance and to increase the overall protein sequence coverage. Additionally, test of SP3 magnetic beads-based method for protein digestion and buffer exchange

• Optimization of MS and MS/MS acquisition parameters to enhance the peptide fragmentation and identification, as well as the relative quantification of post-translational modifications (PTMs)

• Perform a method feasibility study to assess robustness and reproducibility of the optimized workflow according to the ICH Q2(R2) guidelines, including but not li: sensitivity, reproducibility, accuracy, specificity, and robustness.

The optimization will be based on an existing rPepmap workflow using 1-2 mAbs as model proteins. Samples will be measured on a Vanquish UPLC coupled to a Fusion Orbitrap MS system with the capability of performing high-energy collisional dissociation (HCD) and electron transfer dissociation (ETD).

What you’ll bring to the role:

• Postgraduate in analytical chemistry, chemical biology, biochemistry or related fields.

• Theoretical knowledge of mass spectrometry is essential, practical experience in mass spectrometry, instrumental analysis and/or protein (bio-)chemical analysis is of advantage

• Very good knowledge of the English language, both spoken and written

• Flexible, committed and self-organized working style is required

We offer a modern, equipped laboratory, cooperative research work in an interdisciplinary setting and international team as well as comprehensive support.

Why Sandoz?

Generic and Biosimilar medicines are the backbone of the global medicines industry. Sandoz, a leader in this sector, touched the lives of almost 500 million patients last year and while we are proud of this achievement, we have an ambition to do more!

With investments in new development capabilities, state-of-the-art production sites, new acquisitions, and partnerships, we have the opportunity to shape the future of Sandoz and help more patients gain access to low-cost, high-quality medicines, sustainably.

Our momentum and entrepreneurial spirit is powered by an open, collaborative culture driven by our talented and ambitious colleagues, who, in return for applying their skills experience an agile and collegiate environment with impactful, flexible-hybrid careers, where diversity is welcomed and where personal growth is encouraged! 

The future is ours to shape!

Commitment to Diversity & Inclusion:

We are committed to building an outstanding, inclusive work environment and diverse teams representative of the patients and communities we serve.

Hiring decisions are only based on the qualification for the position, regardless of gender, ethnicity, religion, sexual orientation, age and disability.

The law provides for severely disabled / equal applicants the opportunity to involve the local representative body for disabled employees (SBV) in the application process. If you would like to request this, please let us know in advance as a note on your CV.

Join our Sandoz Network:

If this role is not suitable to your experience or career goals but you wish to stay connected to learn more about Sandoz and our career opportunities, join the Sandoz Network here:

#Sandoz

Skills Desired

Summary

• Location: Holzkirchen (Hexal AG) (Sandoz)
• Type: Full time